Radioimmunoassay of Plasma Ligandin

Radioimmunoassay measurement of urinary ligandin excretion in nephrotoxin-treated rats.

[Radioimmunoassay of plasma cortisol].

A radioimmunoassay for the determination of plasma cortisol is described. The antiserum against cortisol was produced by the immunization of rabbits with cortisol21-BSA synthesized from cortisol via hemisuccinate derivative. The antiserum obtained had high affinity and high specificity for cortisol. 0.002 ml of plasma diluted with water was used for the assay. After 1,000 dpm of tritiated corti...

Radioimmunoassay of Human Plasma Thyrotropin.

Many pituitary hormones possess partial or complete immunological species specificity. Earlier reports suggested that thyrotropin (TSH) did not have such specificity since the biological activity of human pituitary or plasma TSH could be neutralized by antisera to bovine TSH in doses similar to that which blocked the biological activity of bovine TSH (2, 3). However, recent studies have shown t...

Radioimmunoassay of cholecystokinin in plasma.

A sensitive and specific radioimmunoassay for cholecystokinin (CCK) has been developed. Synthetic unsulfated carboxy-terminal fragment, CCK-8, was radioiodinated by the conventional Chloramine-T method. Antibodies were raised against sulfated CCK-8 covalently coupled to bovine thyroglobulin via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. By purification, highly immunoreactive 125I-labeled CC...

Radioimmunoassay for dexamethasone in plasma.

Until recently there has been no adequate clinical method for precisely measuring dexamethasone in blood. Studies of therapeutic dexamethasone concentrations and of its metabolism in plasma have relied on the use of radioactive dexamethasone (1-3). We report here an immunoassay in which antisera obtained from rabbits immunized with a conjugate of dexamethasone and bovine serum albumin (BSA) are...